WebMay 15, 2008 · Use of morpholinos in frogs, zebrafish and other organisms The first descriptions of the use of MOs in developmental biology research were made eight years ago ( Ekker,2000; Heasman et al.,2000; Nasevicius and Ekker,2000 ), and since then the research community has adopted them with enthusiasm. WebMorpholinos. The use of antisense morpholino oligonucleotides (morpholinos) in zebrafish was first demonstrated to be an effective method of temporary loss of function in 2000.75 Morpholinos, as chemically modified oligonucleotides, effectively bind target mRNA and block translation thereby knocking down expression.
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