Web8 May 2013 · Strains . BJ3505 and DKY6281 were used for fusion assays (Table 1) ().YCF1 was deleted from BJ3505 and DKY6281 by homologous recombination with the kanMX6 cassette using PCR products amplified from pFA6a-kanMX6 with homology flanking the YCF1 coding sequence. The PCR product was transformed into BJ3505 and DKY6281 by … WebThe strains BJ3505ΔSuc2 and BJ3505 were transformed with plasmids YEpFLAG-1, YEpMEL1, and YEpMEL1His, by a modification of the lithium acetate method (Chen et al., …
HIV-1gp160截短蛋白在酿酒酵母中的表达-杨坤宇李少伟张毅李庶 …
Web15 Mar 2003 · The plasmid was transformed into yeast strains BJ3505 and DKY6281 using the URA3 marker. For the construction of Vtc3-GFP, GFP was chromosomally integrated … WebThe strain BJ3505 carries the wild-type PHO8 gene but lacks genes encoding the major vacuolar proteases that activate the Pho8p phosphatase. Thus, vacuoles isolated from … stephon okibedi
Bioconversion of Beet Molasses to Alpha-Galactosidase …
Strain BJ3505 transformed with YEp MEL1 His was grown for 96 h in 1 L YPHSM before being centrifuged (7000 rpm for 10 min, 4 °C), and the supernatant was filtered through 0.45 µm nitrocellulose membrane filters (Millipore) before being dialysed and concentrated by tangential filtration (TFF, Millipore) … See more To select the best secretion and purification system for the ScAGal protein, different variants of the MEL1 gene were expressed in strain … See more Cell-free BJ3505/YEpMEL1His culture medium was concentrated and partially purified by ultrafiltration. The extracellular protein was then … See more ScAGal not only has strong resistance to treatment with all the proteases tested, but also a higher enzymatic activity in their presence (Fig. 3a). … See more In order to assess the best method of conserving the enzyme, a stability study was carried out in cold and environmental conditions (Additional … See more Webacidocalcisome phosphate homeostasis Introduction Phosphate is a limiting factor for the growth of living organisms. It is mainly taken up by the cells as P i and incorporated in biological molecules, including ATP, nucleic acids, and phospholipids. WebThe coding sequence of truncate gp160 was amplified by polymerase chain reaction (PCR) and cloned into yeast expression vector YEpFLAG-1 to construct plasmid o stephon marbury yahoo