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Strain bj3505

Web8 May 2013 · Strains . BJ3505 and DKY6281 were used for fusion assays (Table 1) ().YCF1 was deleted from BJ3505 and DKY6281 by homologous recombination with the kanMX6 cassette using PCR products amplified from pFA6a-kanMX6 with homology flanking the YCF1 coding sequence. The PCR product was transformed into BJ3505 and DKY6281 by … WebThe strains BJ3505ΔSuc2 and BJ3505 were transformed with plasmids YEpFLAG-1, YEpMEL1, and YEpMEL1His, by a modification of the lithium acetate method (Chen et al., …

HIV-1gp160截短蛋白在酿酒酵母中的表达-杨坤宇李少伟张毅李庶 …

Web15 Mar 2003 · The plasmid was transformed into yeast strains BJ3505 and DKY6281 using the URA3 marker. For the construction of Vtc3-GFP, GFP was chromosomally integrated … WebThe strain BJ3505 carries the wild-type PHO8 gene but lacks genes encoding the major vacuolar proteases that activate the Pho8p phosphatase. Thus, vacuoles isolated from … stephon okibedi https://craftach.com

Bioconversion of Beet Molasses to Alpha-Galactosidase …

Strain BJ3505 transformed with YEp MEL1 His was grown for 96 h in 1 L YPHSM before being centrifuged (7000 rpm for 10 min, 4 °C), and the supernatant was filtered through 0.45 µm nitrocellulose membrane filters (Millipore) before being dialysed and concentrated by tangential filtration (TFF, Millipore) … See more To select the best secretion and purification system for the ScAGal protein, different variants of the MEL1 gene were expressed in strain … See more Cell-free BJ3505/YEpMEL1His culture medium was concentrated and partially purified by ultrafiltration. The extracellular protein was then … See more ScAGal not only has strong resistance to treatment with all the proteases tested, but also a higher enzymatic activity in their presence (Fig. 3a). … See more In order to assess the best method of conserving the enzyme, a stability study was carried out in cold and environmental conditions (Additional … See more Webacidocalcisome phosphate homeostasis Introduction Phosphate is a limiting factor for the growth of living organisms. It is mainly taken up by the cells as P i and incorporated in biological molecules, including ATP, nucleic acids, and phospholipids. WebThe coding sequence of truncate gp160 was amplified by polymerase chain reaction (PCR) and cloned into yeast expression vector YEpFLAG-1 to construct plasmid o stephon marbury yahoo

Saccharomyces Genome Database SGD

Category:The Yeast Vacuolar ABC Transporter Ybt1p Regulates

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Strain bj3505

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Web12 Sep 2012 · Δ BJ3505 and DK6281 strains. ... (60 µl) contained 20 µg vacuoles from BJ3505 backgrounds, fusion reaction buffer, 10 µM CoA, 283 nM Pbi2 (inhibitor of Proteinase B), and 150 nM of the Ca 2 ... Web1 Mar 2000 · A BJ3505 wild-type culture was processed for vacuole purification . An aliquot of spheroplasted yeast cells was lysed by boiling in SDS-PAGE loading buffer, and the …

Strain bj3505

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Web1 Sep 1999 · One strain (BJ3505) has a deletion in the gene encoding the major vacuolar proteases and therefore accumulates catalytically inactive pro-alkaline phosphatase. The …

WebUsing different Rhizopus sp. strains as fermentation agents, this paper describes a global strategy to identify interactions between cultivation parameters (pH 4.75–7.75, 7.5–82.5 g/l glucose ... Web10 Oct 2024 · The aim of this work has been to optimize the recombinant production and further characterization of α-galactosidase of Saccharomyces cerevisiae. Results: The …

WebBJ3505 cells were grown in YEPD to stationary phase, and then suspended in 340 ~1 of 0.1 M sodium phosphate buffer (pH 7.0) and treated with 10 ~1 EMS for 60 min at 30°C; the survival rate was ... teinase-deficient strain, BJ3505, was mutagenized and cells that did not accumulate AB’s in the vacuoles under ... WebTheYeastATP-bindingCassette(ABC)TransporterYcf1p EnhancestheRecruitmentoftheSolubleSNAREVam7pto VacuolesforEfficientMembraneFusion* Receivedforpublication,November29 ...

Web1 Dec 2003 · Batch fermentations were carried out to examine and compare the patterns of cell growth and sugar metabolism for the two recombinant S. cerevisiae BJ3505 strains, δXR and δXR/STH. As shown in Fig. 1, the expression of the STH gene did not make significant changes in glucose utilization, cell growth, ethanol accumulation, and xylitol …

Web1 Mar 2000 · Yeast Strains and Genetic Manipulations. Saccharomyces cerevisiae strains BJ3505 (MATa pep4::HIS3 prb1-Δ1.6R HIS3 lys2-208 trp1-D101 ura3-52 gal2 can) and DKY 6281 (MATa leu2-3 leu2-112 ura3-52 his3-D200 trp1-D901 lys2-801 suc2-D9 pho8::TRP1) were used. The VAM2 and VAM6 genes were disrupted in S.cerevisiae BJ3505 by … stephon newkirk accountingWeb15 Aug 2000 · Frozen BJ3505 vacuoles (300 μg) were diluted to 1.5 ml with PS buffer (see Materials and Methods) and sedimented (13,000 × g, 10 min, 4°C). Vacuole pellets were … pipe for blowing bubblesWeb1 Mar 2024 · The strains BJ3505 1 Suc2 and BJ3505 were transformed with. plasmids YEpFLAG-1, YEp MEL1, and YEp MEL1 His, by a. modification of the lithium acetate method (Chen et al., 1992). stephon ruddWeb1 Aug 2000 · Vacuole Isolation. Vacuoles were isolated from yeast strains BJ3505 and DKY6281 as described ().Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of glutathione S-transferase (GST)-Ypt7p association and for immunoprecipitation experiments. Spheroplast pellets were resuspended in an equal … pipe for cud chewedWebVacuoles were isolated from yeast strains BJ3505 and DKY6281 as described (30). Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of … stephon smith crainWeblated from two yeast strains that express a pro-enzyme (pro-ALP, strain BJ3505) or the respective maturase (proteinase A, strain DKY6281). Upon fusion, the maturase cleaves … stephon mulberryWebThe protein Ixr1p fused to the FLAG ™ peptide at the carboxyl-terminal end was expressed in the yeast strain BJ3505 . Cells were grown at 30 °C beyond 48 h in YPHSM medium (8% Bacto peptone, 1% yeast extract, 3% glycerol and 1% dextrose) until OD 600 nm 6.0, coinciding with the maximum expression. Cells were lysed in TBS buffer (50 mM Tris ... pipe for cud chewed into mush