site stats

Hygromycin selection vector

WebPlasmid vector for blunt-end cloning of PCR-products. Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products. Engineering FRET constructs using CFP … WebCRISPR/Cas mediated genome editing is a revolutionary approach for manipulating the plant genome. However, the success of this technology is highly dependent on selection of a specific vector and the other components. A plant-specific CRISPR/Cas vector usually consists of a Cas gene, target-specific gRNA, leader sequence, selectable marker gene, …

pTK-Hyg Vector - Scientifix

WebMinimal Promoter-Driven Firefly Luciferase Vectors with Hygromycin Selection Part Numbers: E8441, E8451, E8461 Clone a Response Element Upstream of a Minimal Promoter and Luciferase Reporter Gene Minimal promoter (minP) helps drive expression of luciferase when a response element is cloned upstream http://cell-lines.toku-e.com/Cell-Lines_950.html the box by sandra https://craftach.com

Hygromycin B from Streptomyces hygroscopicus - Sigma-Aldrich

Web20 aug. 2024 · To achieve this, we introduced a one-nucleotide deletion into the original pcDNA5/FRT vector, which was necessary for correct expression of the hygromycin resistance gene from the promoter of the ... Web8 feb. 2008 · The selection vectors, pCoHYGRO and pCoBLAST, are commercially available using hygromycin-B and blasticidin S, respectively. Recently, we generated a plasmid, pCoPURO, for selection of transfected S2 cells using puromycin, which allows significant acceleration of the selection time. Although co-transfection of the selection … WebTransfection and selection data of 293 cells by various antibiotics in differing media. Login Researcher's Registration. Cell-Lines; Plasmid/Vector; Antibiotics; Home ... Hygromycin B; 100μg/ml; 1360: 293: pCDNA1neo; Geneticin (G418) 500μg/ml; 1361: 293: pcDNA3; pcDNA3-HA-SSAT; pcDNA3-HA-isSSAT; the box by roddy rich

CHO-K1 cells Cell-culture Database - TOKU-E

Category:Addgene: Vector Database - pcDNA3.1/Hygro(+)

Tags:Hygromycin selection vector

Hygromycin selection vector

Hygromycin B from Streptomyces hygroscopicus - Sigma-Aldrich

Web23 feb. 2024 · Resistance to hygromycin B is an important dominant selectable marker in fungal transformation. Our goal was to improve vectors for hygromycin selection by … Web1 feb. 2024 · The resultant protoplasts were transformed with pAsp shuttle vector. Transformed protoplasts were regenerated into hyphal colonies under hygromycin …

Hygromycin selection vector

Did you know?

WebUsed as a control vector for the NanoLuc® fusion vectors Includes an optimized NanoLuc® luciferase gene that codes for a reporter protein with a shorter half-life ( NlucP) Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Size 20μg Catalog number selected: N1411 $ 435.00 WebThis project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical …

Web4.3 FACS sorting and monoclonal selection of positive cells (optional) While Hygromycin-resistant bulk cells are good enough for initial analysis, FACS sorting followed by … WebThe hygromycin resistance gene is provided under regulatory control of four different promoters, and an IRES, and each plasmid can be further to modified to include your gene of interest if required.This plasmid set has been designed to be compatible with a range of cloning techniques.

Web12 apr. 2024 · Methods: In this study, broccoli, a worldwide Brassica crop, was used to investigate the effects of genotype, explant type, concentration of hygromycin B used … WebVector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's …

Web6 nov. 2006 · Selection of kanamycin-resistant transformants. When wild-type and kanamycin-resistant seedlings growing on medium containing kanamycin (50 μg ml-1) were examined after the 2-d dark period, all cotyledons appeared yellow and closed, and hypocotyls were approximately 0.8–1.0 cm in length.A 24-h light period was sufficient for …

Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique Eco RI, Pst l, and Sac ll sites in the coding … the box cafe plymouthWebThe hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers … the box californiaWebHygromycin . Anne M. Carroll, James A. Sweigard and Barbara Valent-Central Research and Development, E.I. Dupont de Nemours and Co., P.O. Box 80402, Wilmington, DE … the box cafe pearland menuWeb10 apr. 2024 · VectorBuilder offers a variety of drug-selection markers on its vectors, such as puromycin (Puro), neomycin (Neo), hygromycin B (Hygro) and blasticidin (Bsd). In … the box cafe jersey cityhttp://cell-lines.toku-e.com/Cell-Lines_31.html the box can hold all my clothesWebHygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco™ Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 … the box can contain no moreWebThis project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical … the box canes