Fastgene gel/pcr extraction kit
WebJan 18, 2024 · The barcoded PCR products were mixed in equal amounts and then purified using FastGene Gel/PCR Extraction Kit (Nippon Genetics, Germany). The volume of the mixed and purified PCR products was adjusted to 20 to 30 ng/μL. The library was prepared using Ligation Sequencing 1D kit (SQK-LSK108_109; ONT, Oxford, UK) and NEBNext … WebProduct Details. The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA …
Fastgene gel/pcr extraction kit
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WebDNA fragments purified with FastGene Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, … WebApr 13, 2024 · The plasmids used comprised antibiotic selectable markers and were designed according to following strategy: Plasmids were digested with appropriate restriction enzymes (all NewEngland Biolabs). The intentional DNA-fragments were separated by gel-electrophoresis and extracted using the FastGene Gel/PCR Extraction kit (Nippon …
WebThe FastGene® Gel/PCR Extraction Kit should be stored dry at room temperature (15-25°C). Under these conditions, the kit is stable for up to 12 months without showing any … WebMar 23, 2024 · The PCR products from the cDNA of the GATA2 gene were purified by cutting the band of the expected size from 1.5% agarose gels and eluting it with the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan).
WebJan 1, 2010 · The PCR product, which was purified using a FastGene Gel/PCR extraction Kit (Nippon Genetics, Tokyo, Japan), was inserted into the T-Vector pMD20 (Takara Bio, Shiga, Japan). ... WebFastGene Gel/PCR Extraction Kit. DNA-spin Plasmid Purification Kit. Fast DNA-spin Plasmid Purification Kit. G-spin Total DNA Extraction Kit. Let us help you find the right product. Get Help. About. Bulldog Bio is a full-service provider of instruments, tools, and reagents to life science researchers in North America. We make sure that you’ll ...
WebKactus Biosystems. KACTUS is a biotech company that specializes in providing top-notch protein and enzyme products and customized services for a range of fields including antibody drug discovery, immunotherapy, gene therapy, and mRNA therapeutics. Our protein engineering platform is based on advanced bioinformatics structural analysis and …
WebMar 26, 2024 · In order to verify the breakpoint location, analysis of the PCR amplicon in 1% agarose gel electrophoresis was performed and the DNA fragment was extracted using a FastGene Gel/PCR Extraction Kit (Nippon Genetics). ipl edition 2021WebThe reactions were run on the 7900HT Fast Real-Time PCR System (Thermo Fisher Scientific) with the following PCR cycling conditions: 2 min 50°C; 10 min 95°C; 45 x (15 sec 95°C, 1 min 60°C). Identical thresholds were set for all runs for comparison across assays. Mean Cq values from the 50 ng dilution of Universal Human Reference cDNA are shown. ipl entryWebJun 18, 2024 · Purify the remaining PCR products using the FastGene Gel/PCR Extraction Kit (Nippon Genetics) and elute the PCR products by adding 25 μL ddH 2 O to the center of the kit’s spin column. The purified PCR products can be used for a T7 Endonuclease I (T7EI) assay or Sanger DNA template sequences. j. For the analysis of indel mutations, … orangutan borneo photosWebCatalog number: K0691. Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE … ipl english commentatorsWebPCR purification kits and reagents allow for the rapid clean up of PCR products following reactions such as PCR. Various ready-to-use products offer fast and simple protocols in both spin and vacuum formats that remove impurities such as primers, unincorporated nucleotides, buffers, and enzymes. This process is ideal for downstream applications ... ipl englishWebFastGene® Gel/PCR Extraction Kits are designed for the extraction of DNA from agarose gels and for the purification of PCR products. DNA fragments purified with FastGene® … ipl england playersWebDec 14, 2024 · RT-PCR and cDNA purification. ... Amplified products were then separated by agarose gel electrophoresis and products of 2kbp were purified using the FastGene Gel/PCR Extraction Kit (NIPPON ... ipl earnings 2021