Bodipy-labeled palmitate
WebOct 15, 2024 · BODIPY-labeled palmitate (BODIPY FLC16; Invitrogen) was used in conjunction with flow cytometry for uptake experiment. An ELISA kit for mouse IL-2, TNF-α, and IFN-γ were purchased from Abcam (Cambridge, U.K.). WebAug 6, 2024 · To measure FFA uptake in skeletal muscle cells, we adopted a BODIPY-palmitate protocol [ 26] for cultured skeletal myoblasts. L6 GLUT4myc WT cells were grown on 96-well plates overnight at a density of 1.6 × 10 4 cells/well and, following serum-starvation, treated with insulin or ionomycin as described above.
Bodipy-labeled palmitate
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WebMar 1, 2013 · In order to examine if hydrogen could influence FFA uptake, cells were treated with a BODIPY-labeled fluorescent fatty acid analog, BODIPY FL C16, for 15 min after treatment with or without hydrogen for 24 h. After washing, intracellular fluorescence was … WebAug 14, 2024 · d Bodipy 493/503 staining in resting ... supplemented with 10 mM 13 C-palmitate for 18 h, after which they were rinsed with cold 0.9% NaCl and extracted using 0.2 mL of 80% MeOH on dry ice, and ...
WebThe spectral properties of BODIPY FL dye–labeled phospholipids are summarized in Spectral properties of some lipid probes—Table 13.2. Unlike the nitrobenzoxadiazole (NBD) fluorophore, the BODIPY FL and BODIPY 500/510 fluorophores are intrinsically lipophilic and readily localize in the membrane's interior. WebWe used boron dipyrromethene difluoride (BODIPY)-labeled palmitate to show that leptin (60 nM) caused a time-dependent (0-60 min) increase in fatty acid uptake in L6 myoblasts. Quantitative analysis using 3H-palmitate showed that pre-incubation with insulin (100 nM, 24 h) prevented stimulation of fatty acid uptake by leptin.
WebJun 3, 2024 · Medical Definition of Palmitate. Palmitate: An antioxidant and a vitamin A compound that is added to low-fat and fat-free milk to replace the vitamin content lost … WebTo test whether ALL cells take up adipocyte-derived FFA, we differentiated 3T3-L1 adipocytes in the presence of the fluorescent-labeled palmitate analogue, BODIPY …
WebMay 19, 2024 · A mask following the shape of the lymphatic vessel (LYVE1+ staining) was applied to isolate the lipid droplets (LDs) signal (BODIPY 493/503) coming from the LYVE1+ lymphatic vessel (dashed...
WebApr 28, 2016 · While the human placenta must provide selected long-chain fatty acids to support the developing fetal brain, little is known about the mechanisms underlying the … covid test for royal caribbean cruisesWebWith its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids. We recommend dissolving in high-quality anhydrous DMSO to attain a stock concentration of 1 to 10 mM. For Research Use Only. Not for use in diagnostic procedures. brick printing machineWebJun 1, 2007 · The BODIPY-labeled fatty acid analogues are a useful addition to the tools employed to study the cellular uptake and metabolism of lipids. In this study, we show that BODIPY FL C(16) binds to ... covid test for sale at walgreensWebWe used boron dipyrromethene difluoride (BODIPY)-labeled palmitate to show that leptin (60 nM) caused a time-dependent (0-60 min) increase in fatty acid uptake in L6 … covid test for seniorsWebBODIPY-Palmitate is a fluorescently tagged form of palmitic acid (Item No. 10006627) that displays excitation/emission maxima of 488/508 nm, respectively. 1,2 It has been used to … covid test for symptomatic peopleWebBODIPY dyes are unusual in that they are relatively nonpolar and the chromophore is electrically neutral (Figure 1.4.2). These properties tend to minimize dye-induced … ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for t… covid test for thailand entryWebApr 14, 2024 · This finding was confirmed by using alkyne-labeled palmitic acid (alkyne-palmitate), which can be detected upon click reaction with an azide-coupled fluorescent reporter (Gaebler et al., 2016) ... BODIPY-labeled FAs have been previously used to study lipid trafficking and can be esterified into neutral lipids and stored in LDs (Herms et al., ... covid test for spanish holiday